RNA

Part:BBa_K3907003:Design

Designed by: Laura Keffer-Wilkes   Group: iGEM21_Lethbridge_HS   (2021-10-12)


siRNA_scramble against Clpp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 330
    Illegal PstI site found at 344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 330
    Illegal PstI site found at 344
    Illegal NotI site found at 337
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 330
    Illegal PstI site found at 344
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used IDT custom siRNA generator https://www.idtdna.com/site/order/designtool/index/DSIRNA_CUSTOM


Source

BBa_K39087002

References